Composite

Part:BBa_K1615048:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-01)


Heroin Esterase fused to CBDcenA+Linker in RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 21
    Illegal BamHI site found at 777
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion done using RFC25.


Source

Heroin Esterase from Rhodococcus erythropolis. CBDcenA from Cellulomonas fimi.

References